جامعة الكوت - University of kut - بحوث

السنة	عنوان البحث	نشر البحث
2023	Effects of Acrylonitrile Toxicity in Female Ovary and Teratological Abnormalities in Mice	Egyptian Journal of Veterinary Sciences
EJVS_Volume 55_Issue 5_Pages 1409-1415.pdfACRYLONITRILE (ACN) is recognised for its carcinogenic and toxic properties, butits impact on female reproductive health in mammals is poorly understood. This studyaimed to elucidate the chronic zoopathological effects of ACN on ovarian functionand reproductive hormones in female albino mice (Mus musculus), and assess its teratogeniceffects in offspring. A total of 70 female albino mice were randomly divided into three groupsas follows: Group 1 (n=20) servedas a negative control, Group 2 (n=20) administrated ACN at10.17 mg/kg BW (1/10 of the oral ACN LD50) twice a week orally for 120 days, Group 3(n=30) administrated the same dose of ACN three times a week orally for 120 days. On days45, 90, and 120 afterACN exposure, blood samples were collected for to evaluate serum levelsof luteinizing hormone (LH), follicle-stimulating hormone (FSH), and oestradiol (E2) usingenzyme-linked immunosorbent assay (ELISA) kits. After 120 exposure, Group 4 (n=12) werebredwith adult males (n=6) to study teratogenic outcomes. The study found that there weresignificant reductions at all time points in LH, FSH, and E2 levels (P<0.05) of ACN-treatedgroups compared with the control, with Group 3 generally showing the lowest levels.Histopathological analysis revealed necrosis, cystic changes, apoptosis, and haemorrhage in theovaries of Groups 2 and 3. Group 3 offspring exhibited multiple congenital defects includingdwarfism, rough skin, limb malformations, and anotia. All neonates from this group diedpostnatally, exhibited body and tail abnormalities, and presented with kyphosis. In conclusion,ACN exposure significantly impairs reproductive hormone levels and ovarian morphology,leading to severe teratogenic effects. The results of this study indicate that ACN exposure hassignificant adverse effects on female reproductive function in mice, characterized by reductions