جامعة الكوت - University of kut

السنة	عنوان البحث	نشر البحث
2024	ROLE OF CYTOTOXIC T LYMPHOCYTE IN ADENOVIRUS INFECTION	European Journal of Medical Genetics and Clinical Biology
The successful development of adenovirus vectors for vaccines and gene therapy will require a better understanding of the host immune response. Using the ELISPOT assay to measure IFN-gsecreting CD8þ cells, we identify immunodominant epitopes of the adenovirus hexon and DNAbinding protein in BALB/c and C57BL/6 mice. The T-cell response to the intramuscular administration of adenovirus serotype 5 peaks within a few weeks and gradually declines but is still detectable after 12 weeks. A second administration did not substantially increase the number of reactive T cells. The CD8þ T-cell response was also similar between wild type and E1-deleted adenovirus. When B-celldeficient mice were injected with adenovirus encoding the gene for secreted alkaline phosphatase, sera phosphatase activity was reduced more quickly in mice pre-exposed to adenovirus. These results add to the evidence that cell-mediated immunity is a substantial barrier to therapeutic adenoviral vectors and provide more quantitative tools to measure cellular immune responses to adenovirus. Keywords: Adenovirus vectors , ELISPOT assay , T-cell response , BALB/c mice.

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