جامعة الكوت - University of kut - بحوث

السنة	عنوان البحث	نشر البحث
2016	Qualitative and Quantitative Determination of Anti-Cancer Drug (Vincristine) in Catharanthus Roseus by High Performance Liquid Chromatography and Qualitative Identification using other Molecular Spectra Instruments.	Research Journal of Pharmaceutical, Biological and Chemical Sciences
SHIMADZU Preparative-High-Performance Liquid Chromatography was used for qualitative and quantitative estimation of vincristine in aqueous extracted of Catharanthus Roseus plant; (20µl, 2µg/ml)standard vincristine injected and measured at reference conditions to set and fix the real retention time (RT), then 20µl of extracted plant sample was injected and measured at the same conditions. Conditions of separation were; acetonitrile: phosphate buffer: 0.02M sodium dihydrogen phosphate (20:75:5 ml) as isocratic mobile phase, column C18-ODS (25cm x 4.6 mm x 5µm), 20 µl injection volume of sample, and flow rate 1.2 ml/min at 30ºC through 254 nm wave length UV detector Spectrophotometer. An intensive analysis conducted on aqueous, alcoholic extract and dry powder of plant leaves using modern and sophisticated instruments. Alcoholic extraction used for analysis by UV-VIS Spectrophotometer; transparent disk of potassium bromide and dry powder of plant leaves used for analysis using Infra-Red, while dry powder of Catharanthus Roseus leaves used for direct analysis using Attenuated Total Reflection - Fourier Transform Infrared Spectroscopy.Quantitative results obtained from HPLC shows that C. Roseus contain good acceptable concentration of vincristine drug up to 148 µg/gm. Qualitative results obtained from HPLC, UV-VIS, IR, ATR FTIR shows match the effective groups of pure standard vincristine and alcoholic extract, dry powder of C. Roseus plant.