جامعة الكوت - University of kut - بحوث

السنة	عنوان البحث	نشر البحث
2021	CYTOTOXIC AND IMMUNOMODULATORY EFFECTS OF RICINUSCOMMUNISLEAVES EXTRACT	Turkish Journal of Physiotherapy and Rehabilitation
To evaluate the cytotoxic effects, isolated PBMNs and Vero cell line were grown in RPMI 1640 culture medium supplemented with 10% fetal bovine serum (FBS) were treated with the ethanolic and aqueous plant extracts at different concentrations. Cells viability measured via MTT assay after an incubation period of 24 hours. The isolated PBMNs also treated with two concentrations of aqueous leaves extract of Ricinuscommunis to evaluate its immunomodulatory effects by measuring the levels of cytokines produced by PBMNs using ELISA technique. Results: Unlike theAqueous extract, ethanolic extract showed a significant cytotoxic effect at all concentrations tested on Vero cell line and no effect on the viability of PBMNs. Aqueous extract showed a significant decrease in viability of PBMNs only at (1000 and 500) µg/ml. Aqueous leaves extract showed significant suppression of IL-17 secretion at a high concentration as compared to the negative and positive controls. Compared to the positive group, there was a significant decrease in IL-10 and IL-4 production at both concentrations, while INF-γ levels significantly reduced only at higher concentration.Conclusion: The aqueous leaves extract has cytotoxic effects on Vero cells and PBMN only at the higher concentrations, while ethanolic leaves extract has cytotoxic effects on Vero cells at all concentration used. R. communis aqueous leaves extract showed a significant decrease in IL-17, IL-10, IL-4 and IFN-gamma. This suggests a potential use of this extract in autoimmune diseases such as asthma, psoriasis, and rheumatoid arthritis.