جامعة الكوت - University of kut - بحوث

السنة	عنوان البحث	نشر البحث
2021	1. THE EFFECTS OF ANDROGENS ON THE GROWTH AND MIGRATION OF PROSTATE CANCER CELL LINE LNCAP	Journal of Physiotherapy and Rehabilitation 32(3)
The most common type of cancer among men is prostate cancer. It is glandular malignant neoplasia,strongly affected by steroid hormones, especially androgens. Some of these hormones interfere with cell proliferation and invasion of cancer cells into surrounding tissue by cell migration that is an initial step in tumor metastasis. Cell migration is involved in numerous pathogenic processes, including cancer metastasis and inflammation. The aim of this in vitro study was to determine the effects of Testosterone,Progesterone, and Estrogen on the viability and migration of LNCaP. Materials and Methods: Androgen-sensitive human prostate cancer line (LNCaP) used; these cells were treated with different concentrations of Testosterone, Estrogen, and Progesterone hormones to assess the impact of hormonal treatment on cells proliferation and migration. Cell viability determines by MTT assay. For the determination of the effect of sex hormones on LNCaP cells migration, mitomycin used to inhibit cells growth, and thenscratches made in the wells, exposed the cell to Testosterone 250ng/ml, Estrogen 50pg/ml and Progesterone 50ng/ml. The migration rate measured by measuring the diameters of the scratches after 24 hours. Results: Testosterone and Estrogen shows a significant increase in cell viability of LNCaP after 24 and 48 hourswhile Progesteroneshows a significant decrease in cell viability after 24 and 48 hours. Estrogen increase cell migration of LNCaP (P<0.05). Conclusion: Testosterone and Estrogen increase the proliferation of prostate cancer cells.Progesterone has an anti-proliferative effect on the LNCaP cell line.Estrogen Promotes cancer cell migration, while Progesterone inhibit cell migration,