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2025 Comparative Study of Toxoplasmosis in Cats and Human Handlers in Wasit Province, Iraq Using Microscopic and Serological Methods Journal of Medical Science, Biology, and Chemistry (JMSBC)
The aim of this study was to identify and diagnose oocysts of Toxoplasma gondii in cats feces, based on microscopic examination were oval in shape and in blood of handlers by using serological diagnosis (indirect IgG ELISA). The period of study was extended from 1st March, 2024 to 28th Feb., 2025, a total of (70 cats’ fecal samples and 92 handlers blood samples) were collected from both sexes, with different ages. Fecal samples were collected from veterinary clinic, while blood samples were collected from medical laboratories in some areas of province of Wasit, Iraq. The microscopic examination revealed Toxoplasma infection rate 1.42 % by direct wet and flotation with sheather’s sugar solution. On the other hand the percentage of infection by Toxoplasma gondii based on the serological diagnosis was recorded to be 17.39 %, the higher infection rate 19.44 % was recorded in the age group <50 years and the high infection rates were recorded 19.51 % in males. While seroprevalences were 9.61 % and 27.5 % in Al-Kut and Al-Nomaniyah areas, respectively, and all groups showed significant differences at (P 0.01). The results showed that the high prevalence in cats could increase the likelihood of the transmission of feces of cats to handlers. Also it is very important to adopt new methods for prevention of cats from toxoplasmosis infection as well as, the promotion and education to the risk of toxoplasmosis for cats.