مسار بولونيا
EN

أ.م.د. غسان جبار خلف العابدي

السنة عنوان البحث نشر البحث
2021 MOLECULAR_DIAGNOSIS_AND_PHYLOGENETIC_ANALYSIS_OF_BABESIA_SPECIES_ISOLATED_FROM_TICKS_OF_INFESTED_CATTLE_IN_WASIT_GOVERNORATE_IRAQ/links/60eb022d0fbf460db8fc63b3/MOLECULAR-DIAGNOSIS-AND-PHYLOGENETIC-ANALYSIS-OF-BABESIA-SPECIES-ISOLATED-FROM-TICKS-OF-INFESTED-CATTLE-IN-WASIT-GOVERNORATE-IRAQ Iraqi Journal of Agricultural Sciences
many domestic and wildlife animals as well as humans resulting in an intraerythrocytic infection, babesiosis (30). The most important species that affect cattle are B. bovis and B. bigemina which present in Africa, America, Asia, and Australia; in addition to B. divergens that distributed in Europe (4, 19). Babesia species undergo a complex unique life cycle to perpetuate their parasitic existence by propagation and to guarantee host-to-host transmission through specialized infective stages (38). This mediated by the combination of two asexual reproduction cycles and one sexual reproduction cycle, which alternate between the vertebrate host and the tick vector (25, 36). In cattle, babesiosis can detect acutely based on the clinical symptoms and confirmed by microscopic examination of blood smears and or molecular assays whereas in chronic infection, serological techniques as enzyme-linked immunosorbent assays in addition to molecular techniques as polymerase chain reaction which demonstrates with a great value (15). Xenodiagnosis that is, detection of Babesia spp. from ticks, either by microscopy, culture in artificial media and animal inoculation, or molecular techniques has been employed for supportive epidemiological evidence in the diagnosis of babesiosis (8). However, microscopic and cultural methods are time-consuming, laborious, give highly variable results, and need for extensive expertise to be performed (27, 37). Based on 18S rRNA, molecular methods have developed as the method of choice for detection of the parasite genomic DNA, and fo