جامعة الكوت - University of kut - بحوث سارة ابراهيم ناهي

السنة	عنوان البحث	نشر البحث
2020	D-optimal design as a useful tool response surface methodology for the optimization of signals from synchronous fluorescence prior to simultaneous determination of avanafil and tadalafil	Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Arapid,smartandsensitivefirstderivativespectrofluorimetricmethodhasbeencarriedoutforthesimultaneous estimation of avanafil and tadalafil either in their pure form, tablet dosage form or spiked human plasma. The measurements of normal emission spectra or synchronous fluorescence intensity of both drugs show severe overlap which hindered their determination using normal fluorescence or synchronous intensity. Therefore, a highly sensitive first derivative synchronous fluorescence procedure was used to resolve this overlap. The methodisbaseduponmeasurementoftheamplitudeofthefirstderivativeofsynchronousfluorescenceintensity of bothdrugsatΔλ=70nmandatsuitablewavelengthof396nmand364nmforavanafilandtadalafil,respec tively. Under the optimum conditions, the linear determination ranges are 50–1800 and 5-400 ng mL−1 with a detection limit of12.93 and1.46ngmL−1foravanafilandtadalafil,respectively.Aresponsesurfacemethodology wasusedforoptimizationusingD-optimaldesignwhichcanbeusedfordeterminationoftheexactoptimumpa rameters specifically designed for this method. Inaddition; itis a good wayto graphicallyclarify the relationship between various experimental variables and the synchronous fluorescence intensity.