مسار بولونيا
EN

لمى حكمت كريم البياتي

السنة عنوان البحث نشر البحث
2018 Validation of three DNA extraction methods for the detection of gram positive and gram negative bacteria
Abstract: BACKGROUND: The first step of any genetic engineering research is DNA extraction and pure DNA is an essential part of the sensitivity of a diagnostic test. There are various methods which extract bacterial DNA from different specimens. The objective of the present study is to compare three DNA extraction methods for gram positive and gram negative bacteria to find out the most suitable of them for PCR assay. METHODS: Using three DNA extraction methods including boiling, phenol-chloroform and commercial kit (Sinaclon, Iran). DNA from two bacterial species, Gram-negative bacterium (E. coli) compared to the Gram-positive bacterium (Clostridium perfringens) were used. Concentration and purity ratio of extracted DNA from both gram positive and gramnegative bacteria using the three different extraction methods was measured. Finally, conventional PCR test was conducted for evaluating the efficiency of the DNA RESULTS: DNA extracted by the commercial kit (Sinaclon, Iran), had the most purity and it was the most reliable method to have appropriate concentration of DNA